In China, ITS+premix can not be imported because of mad cow disease, and other ITS from SIGMA or THERMO SCIENTIFIC manufacturers have different formulas. So could I directly make a recipe like:insulin (6.25ug/ml), transferrin (6.25ug/ml), selenous acid (6.25ug/ml), and linoleicacid (5.35ug/ml), with bovine serum albumin (1.25mg/ml))? or why people always bought ITS+premix instead of making ITS by themselves? I mean people write this formula in their papar, they know the formula, but they don't make ITS themselves. Are there something else behind the formula?
I will really appreciate your reply.THANK YOU.
Yes, there is no reason why you cannot mix your own reagents.
As to why others do not do so, there are usually two reasons: convenience and validation of quality.
Of course, both things come at a price but then we now live in the age of science by kits - increasingly, people buy ready-made bottled sterile PBS and even Saline! ;)
I think it's just because it's more convenient and safer since the ITS can be contaminated during preparing process.
Yes you can do it, we did it several times when the ITS+ was back ordered (which happens from time to time)
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