颖影 Messi

4 Questions 5 Answers 0 Followers

Questions related from 颖影 Messi

Any help about chondrogenic differentiation media? Could I make ITS+premix by myself?

In China, ITS+premix can not be imported because of mad cow disease, and other ITS from SIGMA or THERMO SCIENTIFIC manufacturers have different formulas. So could I directly make a recipe...

15 December 2015 5,920 4 View

Where can one buy type Ⅹ collagen?

Recently I will use the type Ⅹ collagen in my experiment, but I find it difficult to find a company selling it. Could anyone introduce a company to me? I am really eager to get it. Thanks a lot!

03 March 2015 6,706 4 View

Could someone give a p rotocol to extract collagen Ⅱ from cartilage?

I wanna to quantify the collagen Ⅱ in cartilage using Elisa, when I break the cartilage tissue to gain collagen Ⅱ solution. I try to use stator-rotor homogenizer, but there are still some tiny...

12 February 2015 6,343 3 View

Will a lack of nutrient cause fibrosis of a chondrocyte? Can the change of culture medium osmotic pressure result in chondrocyte fibrosis?

This picture is taken after a one-week culture in droplet. Why do the chondrocytes become like this? I guess maybe it's due to a lack of nutrients or the change of osmotic pressure. What do you...

04 September 2014 4,140 5 View