As reported, newly formed β-granules are preferentially secreted whereas aged β-granules mainly undergo degradation via crinophagy or autophagy. Insulin production/secretion imbalance is common in T2DM. I am searching for the methods to measure the life span of insulin granules confirming that aged and non-functional β-granules take up the majority of the insulin storage pool. And approaches detecting the insulin granules recycling also needed.
Thank you for your kind help! Researchers obtained delicate images of the dynamic insulin granule fusion. As refer to equipment limitations, we will rely on two proton microscopy for recording these exocytosis events. I want to put forward an interesting question further: What's the mechanism behind newly formed β-granules are preferentially secreted whereas aged β-granules mainly undergo degradation via crinophagy or autophagy. What‘s the difference between these two granules?( labeled protein or other markers?)
A recent and very nice study that uses a SNAP tag to visualize the preferential secretion of newly synthesized insulin.
http://diabetes.diabetesjournals.org/content/62/11/3687.long
Thanks a lot, David! This is just the article I am searching for. It will be awesome to apply this SNAP tagged visualization to our research. And I will read through it for more details.
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