Droplet PCR in plastic chips always result an unexpected band, any suggestion of possible contaminations and how to improve?

I am doing droplet based digital PCR inside plastic chips. I can get the correct band, but I also consistently get an unexpected band, slightly shorter. I attached a typical gel image here....

18 February 2021 6,618 2 View

How to interpret this LC3 immunoblotting result for the autophagic function?

I performed an immunoblotting with LC3 antibody (please see the image) upon siRNA-mediated knockdown of two different proteins, A and/or B, with or without Bafilomycin A1 treatment. I would like...

20 October 2019 8,517 4 View

How to make soil suspension?

Hi all, I plan to test effects of some chemicals on soil microorganisms. Culture medium is still hard to finish that. Because they are difficult to mix with soil thoroughly, I hope to make...

20 June 2019 2,941 2 View

Progress in integrated utilization of solar energy (Especially for sorption technologies)?

Utilization of solar energy is always one of the meaningful research areas. But most researches are about one or two utilization aspects, such as for cooling, heating, heat storage, power...

03 June 2019 6,983 2 View

Evolution in thermodynamics?

We are planing to summarize some prospective researches to fill the evolution in thermodynamics. I am looking forward to your recommendation of your research or others.

18 March 2019 9,161 18 View

Laser capture microdissection-- tissue just not picked up?

Anyone had experience in Laser capture micro dissection of H&E stained FFPE sections mounted on regular glass? I found that the cutting is OK just the tissues won't be picked up. Only leaving...

11 March 2019 7,137 6 View

How can i proof that the doped atoms in one lattice host occurs alignment?

21 October 2018 8,694 5 View

Is it possible to make emulsion about hundred micrometer in diameter with molecular surfactant?

I am trying to compare nanoparticles and molecular surfactants for hydrate formation process. Pickering emulsion stabled by nanoparticles are usually in hundred micrometer in diameter. So I am...

21 July 2018 7,009 3 View

What is the chemistry related to darkening of CH3NH3PbI3, HC(NH2)2PbI3 precursor solutions, after aging?

29 March 2017 8,288 3 View

What is the highest efficiency of hole-conductor-free perovskite solar cell?

What is the highest efficiency of hole-conductor-free perovskite solar cell? Thanks.

19 January 2017 2,056 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View