Yingyun Gong

6 Questions 6 Answers 0 Followers

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How to reduce stress when sac mouse and harvest tissues?

Our project designed to distinguish different norepinephrine content in mouse liver between groups. Here come to our concerns. how to sac mice without disturbing their physiological sympathetic...

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Could the secreted PCSK9 from HepG2 cells be measured in culture medium?

It is known that PCSK9 is a kind of secretory protein from hepatocytes and serum PCSK9 is measured by ELISA kit. I was wondering whether it be possible to detect the secreted PCSK9 from cultured...

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How do separate lipoprotetins?

I got to know that lipoprotein profiles (not only for cholesterol, but also for triglyceride) could be characterized by an automated gel-filtration system (Eur J Clin Invest 2006; 36 (2): 98–104)....

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Does induced brown adipose tissue mean there is no classical BAT in adults?

I am quite confused about the definition of cold or other stimuli-induced brown adipose tissue. Does it equal to beige adipose tissue? In this case, the "brown fat" we are now talking about is...

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How to define obese or not in a mouse model

In human studies, obese is defined as BMI over 30kg/m^2. What's the universal criteria for different mouse models?

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In what form do plant sterols exist in the bloodstream？

In what form do plant sterols exist in the bloodstream？ In lipoprotein particles or in extracellular vesicles?

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