Any good genomic DNA extraction protocol from cell lines with out using kit?

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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11 August 2024 5,138 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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Could it be a cell culture contamination?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

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Does crude extraction using NaOH and Tris work well with Fungi?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

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06 August 2024 641 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

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Hung King Tiong

I have used the bead extraction method (size 4-8 micrometer) for chr. DNA extraction. It works fine.

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The original protocol can be found at : http://www.sciencedirect.com/science/article/pii/S0167701205001077

Richa Tyagi

thanks so much.

Ricardo Frausto

A method I have used in the past and results in high yield and good quality DNA is Tris-buffered Phenol/Chloroform followed by Ethanol precipitation. Include an RNAse treatment to remove carryover RNA.