I want a gene to express in a pulsed pattern, not continuously, resulting in stimulation intervals. Any expression system can be applied to facilitate this pattern? Thank you for your suggestions!
Use a promoter from one of the circadian rhythm genes? Or you could try a quorum sensing based repressilator.
Sorry, I stuck in other problems these days and forgot to check. Thanks a lot, I'm going to try the circadian genes, I never heard of the repressilator, I just checked, that's fascinating , I'll look into details to see if it can work in my case. Those are precious suggestions, very appreciated!
Perhaps, optogenetics may help you control expression of your gene of interest in a rhythmic and reversible manner rather than conventional systems that rely on conditional promoters.
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