Tao Zhou

4 Questions 7 Answers 0 Followers

Questions related from Tao Zhou

Any expression system can drive a repetitive intermittent gene expression, like a pulsed pattern?

I want a gene to express in a pulsed pattern, not continuously, resulting in stimulation intervals. Any expression system can be applied to facilitate this pattern? Thank you for your suggestions!

07 December 2023 7,703 4 View

When to restimulate human T cells?

If I stimulate the PBMC with coated CD3CD28 mAb, should I transfer cells to uncoated plate after 2-3days of stimulateion to avoid over stimulation? When can I restimulate the cells and how should...

30 November 2017 5,064 0 View

Which CD3/CD28 antibody are good for in vitro T cell activation?

Hi I want to use pre-coated CD3 and CD28 mAb to activate T cells. I just realize there are different types and clones to choose. Based on your experience, which antibody works best？from which...

15 March 2017 1,581 11 View

Should VH be placed in front of VL in FMC63 scFv structure?

I read papers about the structure of CAR vector and find inconsistence in the design of FMC63 scFv. VH is placed in front of VL in some papers while VL is placed in front of VH in other papers. I...

16 January 2017 2,527 2 View