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How can CRISPR-mediated targeted knock-in achieve gene expression levels more than ten times higher than the baseline? Literature reports that lentiviral infection followed by screening for...
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I want a gene to express in a pulsed pattern, not continuously, resulting in stimulation intervals. Any expression system can be applied to facilitate this pattern? Thank you for your suggestions!
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If I stimulate the PBMC with coated CD3CD28 mAb, should I transfer cells to uncoated plate after 2-3days of stimulateion to avoid over stimulation? When can I restimulate the cells and how should...
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Hi I want to use pre-coated CD3 and CD28 mAb to activate T cells. I just realize there are different types and clones to choose. Based on your experience, which antibody works best？from which...
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I read papers about the structure of CAR vector and find inconsistence in the design of FMC63 scFv. VH is placed in front of VL in some papers while VL is placed in front of VH in other papers. I...
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