Renerio Jr. Pelegrino Gentallan

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Questions related from Renerio Jr. Pelegrino Gentallan

Is the term genome applicable to all ploidy levels?

The concept of the term "genome" refers to: in 1920, “the haploid chromosome set, which, together with the pertinent protoplasm, specifies the material foundations of the species” (Lederberg et...

10 October 2018 8,124 6 View

Is there an arbitrary classification for the total phenolic content (%w/w)? How high is high? how low is low? are there any standards in classifying?

I am currently having conduct a study regarding the total phenolic content of a plant extract; however, I cannot determine if a 42.7 mg/g gallic acid equivalent phenolic content is high or not.

08 August 2018 4,323 4 View

Oil content determination using oven dry method?

I want to ask if determining the seed oil content using gravimetric means such as done in the paper below is appropriate. There were no chemicals used. I could not find any other literature...

03 March 2018 7,241 4 View

Does freeze drying and storing plant tissues in -80 deg. C affect the total RNA yield in RNA extraction?

It is because I have very low RNA yield. I was thinking if it is because I use 1-week old freeze dried samples stored in the freezer at -80 degree celsius. Amybe it is better to use fresh samples...

07 July 2014 6,446 10 View

In spectrophotometric analysis, what would a >2.0 260nm/280nm absorbance ratio of an RNA extract indicate?

My hypothesis: Since nucleic acids has a peak absorbance at 260nm wavelength and proteins at 280nm, the ratio of greater than 2.0 is considered relatively pure RNA. Twist: However, literature...

06 June 2014 1,201 4 View

Can qPCR results indicating relatively high gene expressions of desired gene of one cultivar compared to others be a reason for selection?

This is because I'm conducting a gene expression analysis of a certain gene on sweet potato germplasms.

02 February 2014 1,383 0 View