Merissa Olmer

3 Questions 2 Answers 0 Followers

Questions related from Merissa Olmer

Is there a way to clean up this RNA (tissue high in proteoglycans)?

Hello, I've tried numerous ways to extract RNA and I've used methods in the paper below. The supplemental has great suggestions/troubleshooting recommendations. I've tried re-extraction with...

23 August 2021 1,394 0 View

Has anyone run extracted RNA through the Zymo RNA extraction kit again?

We have some RNA that was extracted using the Zymo directzol RNA extraction kit. We used trizol and then did a chloroform phase separation. We see really poor 260/280 and 260/230 ratios. It was...

08 July 2021 2,088 2 View

Having problems with cryosections staying on slides for LCM. Can I coat charged slides and will coating impact RNA quality?

We are trying to use LCM for RNA extraction and our first hurdle that we cannot manage to jump is an issue with the tissues falling off of the slides. We are using positively charged slides for...

23 March 2018 6,600 0 View