How can I increase cDNA concentartion obtained from viral RNA?

I need good quality and quantity of viral cDNA for NGS analysis. Please suggest how can I increase amount of cDNA obtained from viral RNA.

02 February 2021 8,379 3 View

Pcambia 1301 sequencing ?

I got a LB plate of pcambia 1301 binary plasmid from an institute. I performed miniprep to isolate the plasmid. Now I want to sequence this plasmid. What kind of primers and reagents will be...

03 November 2020 6,075 4 View

Can I use gene specific primers to sequence my insert after TA clonning in pcr 2.1 ?

After ligating the insert in TA vector pcr 2.1, transformation and miniprep. I amplify the gene with simple gene specific primers . Now I want to sequence the insert . Which primers would I need...

31 October 2020 7,077 4 View

Is the electrical resistivity of individual multi walled carbon nanotube (MWCNT) different in longitudinal and transverse direction?

There is a lot references in which the anisotropy of CNTs were discussed but I can not find any paper that describe the anisotrpy of an individual MWCNT in electrical resistivity. Is the...

08 September 2020 4,720 2 View

How to correct baseline for Lorentzian noise?

Dear all, Could you please suggest a method for removing background noise which has Lorentzian shape? Best regards, Qurat

30 August 2020 6,907 2 View

What are the best topics for research on environmental informatics, immuno informatics and nano informatics now a days alongwith the best methodology?

best research topics and methodologies on pollulatnts which influence genetic diseases and the treatment through nano technology?

09 August 2020 5,326 1 View

What is the IC50 value of Fluorouracil against HT-144 cell line?

I cannot find the IC50 value of 5-FU against HT-144 cancer cells, as determined via cytotoxicity analysis. I shall be thankful if someone help me with relevant literature.

15 June 2020 4,375 3 View

How to perform leakage radiation assessment from X ray tube with survey meter ?

is it possible to do with just a mini ordinary survey meter ? for a non-separable mAs setting type model, it is said to measure the dose (mSv, mGy) instead of the dose rate (mSv/, mGy/hour). then...

24 February 2020 6,982 5 View

What is the reason behind reduced nanoparticle size after drug loading?

Most of the researches I went through suggest increased crystallite size after being loaded with a drug. However in a unique case, I have observed reduced average diameter after drug loading. Can...

18 January 2020 2,044 11 View

Is there any minimum age limit for conducting survey?

Can simple questionnaire be distributed to 8-12 years old student?

17 May 2019 6,971 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View