what are the mechanism of T cells exhaustion in cell culture,
We usually restimulate the T cell clones every 2 weeks by adding irradiated PBMCs with IL-2 ,PHA to induce their proliferation. does this have an effect in triggering the T cell exhaustion
Have you tried IL-7 and IL-15? I had better results with these cytokines i a short antigen based stimulation and culture. I don't know about maintaining T cells beyond 7-10 days though. Here is a link that might be worth checking out http://www.sciencedirect.com/science/article/pii/S0042682212004539
We routinely use PHA to activate T cells triggering apoptosis when generating EBV transformed B-LCLs. The majority of the T cells typically die off during the third week. You might like to look at "Induction of activation-driven death (apoptosis) in activated but not resting peripheral blood T cells." http://www.jimmunol.org/content/150/10/4338.long
Hi Colin, please state the sources that back up the claim that T cell exhaustion does not exist. Not trying to pick a fight or anything, I am just interested and would love to read those sources.
I would start with Dan Barber's Nature paper, itself a very nice disproof of exhaustion. We discuss it in a review article in a now defunct journal (see: Co-inhibitory molecules: Controlling the effectors or controlling the controllers? G Thangavelu, C Smolarchuk, CC Anderson, Self/nonself 1 (2), 77-88. One may simply argue that exhaustion is the wrong word for the phenomenon. I would be interested if there is data that counters our view.
Colin do you refer to - Restoring function in exhausted CD8 T cells during chronic viral infection? Presumably your comment is based only on the fact that these T cells can then be rescued from exhaustion? Although I would argue that human beings can also be rescued from exhaustion - it does not make them any less exhausted in the first instance no?!
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