@Craig-Rive-2

Craig Michael Rive

1 Questions 10 Answers 0 Followers

Questions related from Craig Michael Rive

Can anyone confirm that the probe in a TaqMan Assay should have a Tm 5-10'C higher than the Forward and Reverse primers and NOT 5-10'C lower?

I just want to confirm that the probe in a TaqMan Assay should have a Tm 5-10'C higher than the Forward and Reverse primers and NOT 5-10'C lower? Given a final concentration of 500nM of primers...

08 August 2014 832 3 View

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