I have a compound that is low molecular weight, antibacterial in nature, and shows absorbance in UV light on a TLC. So how would I characterize it in nature? For example, is it a peptide? or a phenolic compound or something else. Please give me suggestions regarding this.
If you have at least a few mg, you could get quite a lot of information about it by various NMR experiments, with expert help on performing the experiments and interpreting the results. The mass could be determined by mass spectrometry.
Adam B Shapiro, Thank you for your suggestions. I am looking forward to NMR spectroscopy, but before that, I want to gather some characteristics of this compound. Thank you for the suggestion.
Infrared spectroscopy is another method that would give you clues about the chemical structure.
Tole S.B., thank you for your valuable suggestion, I am performing a spectral analysis of the compound but first I have to purify it with HPLC. And I am summarising that first but I will try to do what you said.
Infrared spectroscopy (IR) can be used. Used to identify functional groups in organic compounds. Or use mass spectrometry. Including liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS), which are suitable for different types of compounds and can determine molecular formulas and perform structural analysis.
Raman spectroscopy is another method that can be used to study chemical composition.
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