Hi everyone,
I accidentally left the antibody on the bench for one hour. is that going to effect its binding ability?
Thank you in advance for answering :)
Dear Delilah MH
Its depend on the type of Abs and its stability, antibodies 1 hour are fine at room temperature (for several plant viral AB it works after 3 days with no harm , Usually I stored at 4°C and these work perfectly fine for months), although it depends on the Abs and how it was produced and manufactured; but, generally, 1 hour is not a big time to lose their binding activities. Antibody is like any protein, some can't be frozen, most need to be stored at 2 to 8ᵒC and can last for several months. Antibodies are meant to be stored at -20C , each freeze-thaw cycle lead to reagent be less efficient. Ideally, -20C antibodies have glycerol-like components pre-added and you do not really need to keep them out for too long to thaw. good luck
Pls. find the attached files
https://www.labome.com/method/Antibody-Shelf-Life-How-to-Store-Antibodies.html
http://www.abcam.com/protocols/antibody-storage-guide
http://www.newenglandpeptide.com/support/antibody-care-and-storage
http://www.sciencedirect.com/science/article/pii/S0378517305007076
https://www.anaspec.com/html/antibody_notes.html
http://biology.stackexchange.com/questions/34045/antibodies-stored-in-freezer-instead-of-fridge
http://www.aveslab.com/resources/antibody-storage-recommendations/
http://www.thermofisher.com/1/3/storage-temperature-for-antibodies
https://www.sysy.com/protocols/
http://www.aveslab.com/resources/antibody-storage-recommendations/
Hoping this will be helpful
Regards
Prof. Houda Kawas
Extremely unlikely. If your antibody is that sensitive to temperature, there were likely other things wrong with it in the first place.
I agree with Jordan. Most antibodies are quite stable. Consider that (human) antibodies are optimized to be active in an environment of about 37 °C.
Dear Delilah MH
Its depend on the type of Abs and its stability, antibodies 1 hour are fine at room temperature (for several plant viral AB it works after 3 days with no harm , Usually I stored at 4°C and these work perfectly fine for months), although it depends on the Abs and how it was produced and manufactured; but, generally, 1 hour is not a big time to lose their binding activities. Antibody is like any protein, some can't be frozen, most need to be stored at 2 to 8ᵒC and can last for several months. Antibodies are meant to be stored at -20C , each freeze-thaw cycle lead to reagent be less efficient. Ideally, -20C antibodies have glycerol-like components pre-added and you do not really need to keep them out for too long to thaw. good luck
Pls. find the attached files
https://www.labome.com/method/Antibody-Shelf-Life-How-to-Store-Antibodies.html
http://www.abcam.com/protocols/antibody-storage-guide
http://www.newenglandpeptide.com/support/antibody-care-and-storage
http://www.sciencedirect.com/science/article/pii/S0378517305007076
https://www.anaspec.com/html/antibody_notes.html
http://biology.stackexchange.com/questions/34045/antibodies-stored-in-freezer-instead-of-fridge
http://www.aveslab.com/resources/antibody-storage-recommendations/
http://www.thermofisher.com/1/3/storage-temperature-for-antibodies
https://www.sysy.com/protocols/
http://www.aveslab.com/resources/antibody-storage-recommendations/
Hoping this will be helpful
Regards
Prof. Houda Kawas
I have experience with human autoantibodies. Very stable also at room temperature !
Hi everyone? Does anybody know if an agarose bound lectin should still work if you left it at RT instead of 4degrees Celcius?
Thanks you in advance!
In general peptides bound to agarose are very stable. I can imagine that it is the same for lectins.. 4°C or 25°C is is not very imoprtant .
Depends for how long. The only thing I'd be worried about is microbial contamination. Have you added antimicrobials?
There is no worry about that! My only concern was if the binding would be reduced cause optimal temperature of lectins for binding with proteins is 4•C.
Thanks a lot though!
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Antibodies