Antibiotic range to be tested in Kill curve for CHO-S cells?

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Role of passive components in RF transceiver system design?

I am unable to quantify the role of passive components when it is attached with the antenna. For various frequency bands like L,S,C,X the specifications of the active circuits changes but what...

25 August 2020 1,463 4 View

Question on detection of DNA Protein covalent complexes by dot blot. How to achieve reproducubility?

I am trying to detect DNA-protein covalent crosslinks which are induced by my drug treatment. I am following the RADAR assay protocol. Briefly, I am lysing cells using a buffer containing 6M GITC,...

30 July 2020 949 4 View

What are the artificial intelligence models which can apply to estimate river bank erosion potentiality?

I am working with such kind of models

20 July 2020 7,496 4 View

What are some good reading materials for a course on Introduction to Qualitative Research?

I am designing an outline for an introductory course on Qualitative Research for PhD students, and looking for research papers (conceptual/empirical) that may be recommended to the scholars.

28 May 2019 2,736 5 View

Is there a in-built function in MATLAB for identifying an arbitrary point with a particular voronoi cell?

I have a voronoi cell structure. I also have some arbitrary points. I need to know if there is an in built function in MATLAB to identify which arbitrary point comes under which point's domain of...

09 April 2019 8,770 1 View

What are basic differences between data mining and machine learning?

Can anyone clearly explain the differences between data mining and machine learning and whether a classifier comes under data mining or machine learning (e.g. MLP, SVM etc.)?

31 March 2019 5,888 9 View

Identifying PGE by SEM and XRD. Will it be possible to get more or less same results by the Fire Assay method?

Getting result of SEM and XRD with full positivity in PGE concentration in a gabbro-anorthositic layered complex but not getting full support when analyzed by fire assay method.

14 February 2019 423 3 View

Why do metal nanoparticles produce metal ions in aqueous solutions?

I am working with copper nanoparticles. These nanoparticles are producing Cu2+ ions in PBS. I want to know that whether these ions release is somehow related to the loss of conduction electrons...

06 June 2018 4,977 4 View

Oil is spread on glass and a drop of water is put on it, how to visualize (3D) it using SE FIT or any other software?

Soft condensed matter, SE FIT

31 July 2016 3,102 3 View

Is it possible to do statistical data analysis by Mahalonobis method?

My data comprises of bulk geochemical i.e.,major, trace and REE parameters of granitoids. Mineralogical and norm calculation do not show much variation of data. However, I can correlate data...

13 June 2016 8,414 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Why is there no protein expression but antibiotic resistance in transduced A549 cells with pLVX-TetOne-Puro lentivirus?

Hi, I have been trying to transduce A549 and Calu-3 cells using the Lenti-X Tet-One inducible expression system (TakaraBio). My gene of interest is inserted in a pLVX-TetOne-Puro plasmid and has...

15 February 2021 8,462 3 View

What are the naitve functions of bacterial NRP?

The bacterial NRPs (nonribosomal polypeptides) are famous as clinical drugs such as antibiotics, but is there other native function of these NRPs of bacteria except antimicrobial, because their...

01 February 2021 4,280 1 View

Why I am not getting cultures of DH5 alpha and BL21 cells after transformation?

Even if I get them cultured colonies are very less and almost not transferable and grows very slowly in the media. I checked cells by transforming with control vector. I changed antibiotic also...

06 January 2021 1,013 3 View

Is it normal to use Tris buffer when testing for bacteriostatic properties of isolated compounds?

Hello, to start off I attempting to test the bacteriostatic properties of certain compounds. I bought a kit that contains some of the equipment to perform this analysis, however, in the included...

02 January 2021 2,338 2 View

Is there any database or website from where I can get a list of FDA approved drugs for treatment of certain urinary tract infection?

I want to know all prescription drugs which are FDA approved compounds for infection like Urinary Tract Infections along with their causative agents. Can you guide me about any resource, database...

17 December 2020 4,852 3 View

Does anyone experience E coli lysis while seed culture?

Hi, I am currently working with BL21(DE3)PLySs and PRSET A vector. Out lab frequently transformation and culture E Coli for protein purification. But recently, even though there is colony after...

13 December 2020 9,167 9 View