Sarmishta Majumdar

3 Questions 17 Answers 0 Followers

Questions related from Sarmishta Majumdar

How to carry out a kill curve experiment?

Could someone please help me understand how to carry out a kill curve experiment? This is for antibiotics G418 and Puromycin for CHO cells

06 June 2023 2,287 0 View

How to check if the ATP is hydrolyzed in the ligation buffer? Does the ATP form any white ppt at the bottom of the tube, and dissolves after mixing?

Since my ligation is not working (no colonies in insert: vector plate) and analyzing my results where we could find that my digestion is just fine and same in case of my transformation (lawn in...

20 January 2023 2,622 8 View

Ligation not working, reason?

I have tried to ligate my vector+insert in ratios of 1:3 and 1:5, along with keeping a vector only control. Although, I got colonies post transformation the no. of colonies is more or less same in...

02 January 2023 6,206 21 View