What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Why would one go up and then down with gradient during HPLC?

I think I've seen it previously, but the current example is from this publication https://www.mdpi.com/1420-3049/14/5/1825 where they used the following gradient on HPLC: min ... % MeOH 0 ... 2 20...

09 February 2021 5,422 3 View

How can you alter line thickness in emmip plot (R)?

I am working with R and plotting emmeans graphs, yet I cannot find online how we can change the line type or its attributes using the emmip function. I have two independent categorical variables,...

27 October 2020 9,339 1 View

How to synchronize two stepper motors most optimally?

We need to push a large load (2 tones) using two stepper motors, connected rigidly to the axis (two screws). I'm worried the motors could micro-step out of sync. And cause malfunction. I can see...

21 October 2020 6,363 4 View

How to fix convergence problmes using Mohr Coulomb Plasticity?

Dear colleagues, I am trying to simulate a strip foundation on a cohesionless soil with a friction angle of 30 degrees. However, I obtain convergence problems. I am using a geostatic step. The...

21 October 2020 4,556 2 View

Can you integrate Zotero with this website?

Is it possible to integrate Zotero or any other bibliographic software application with this website application? If not, is it possible to do that with academia, ssrn or Google Scholar? Any...

21 October 2020 4,712 1 View

How to find out, if a recent Sentinel-2 acquisition failed or had a problem?

Hello, I am currently waiting for an image to appear on Scihub and it takes longer than usual (acquisition date 2020-09-22, orbit r122, T33UWR tile actually, but looking for general answer). So it...

23 September 2020 6,842 5 View

Can small law firms adopt AI?

After considerable research, I have found several use cases which can be used to exemplify some implementations of AI on law firms. However, there is clearly a disadvantage for this segment when...

01 September 2020 7,132 5 View

How can i model a shock tube with 2 different gases on Fluent?

Hi, I'm currently trying to study shock tube analysis on fluent and I have modelled a shock tube with a driver section (high-pressure) and a driven section (low pressure). What I want to study is...

22 July 2020 8,162 3 View

How can i make an isopeptide bond in GROMACS ?

Hi everyone I'm new in all the field of molecular dynamics, but i trying to run a SMD of one protein with two isopeptide bond, one in each domain, that prevent my protein stretch. My problem is...

07 July 2020 6,201 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View

During an anti-His capture ELISA has anyone had issues with histidine residues from secondary antibodies non-specifically binding?

Hi, Hope you can help. Currently been running some sandwich ELISA assays where I coat with anti-His, block and then capture His-tag protein and then sera and then anti-bovine monoclonal...

07 February 2021 7,101 1 View

How do I keep my prefixed (PFA) frozen tissue sections from lifting off the slide?

I'm trying to stain mouse lung tissue IHC-IF I fix in 4% PFA overnight immediately upon harvesting tissues Then dehydrate/cryoprotect in sucrose gradients 15% to 30% Then freeze in OCT media...

06 February 2021 2,336 3 View

Antibody dilution for IHQ in OCT frozen tissue is more similar to IHC in Paraffin-embedded samples or to Immunocytochemistry?

31 January 2021 8,678 3 View

Can i re-incubate a WB membrane with a higher solution of the previously used 1ry ab?

I've incubated my western-blot membrane with both primary and secondary antibodies and i just realized at the end that i've used a lower concentration of my primary antibody for my loading control...

27 January 2021 1,034 3 View

How to prepare secondary antibody from lyophilized antibody?

So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...

27 January 2021 5,402 3 View