Can anyone recommend a method for ethylene gas detection in an air sample?

I am looking for a method to detect ethylene gas concentration in a green house. I have tried SPME , Head space and GC-MS analysis but results were not satisfied. So is it better to detect...

10 January 2019 7,832 3 View

Contamination in A549 cell culture?

I am culturing A549 cells, I have noticed there are white color particle like things floating on the media. it was visible to the naked eye also. there was no color change in the media or there...

22 February 2018 8,404 5 View

Why there were many floating cell, when THP-1 cells treated with PMA ?

I have done THP-1 cell differentiation into macrophages. I used RPMI as a media. and PMA 30nM concentration. after 24 hours incubation there were many floating cells. what could be the reason? and...

22 January 2018 3,473 3 View

Could anyone explain me the difference between non-verbal mental age and verbal mental age please?

14 March 2017 8,969 6 View

To do a DNA molecular dynamics simulation using GROMACS do I have to constraint the back bone?

When visualize my simulation using pymol, one strand is going out of the box while other strand is remaining inside. To do the simulation, do i have to constraint the backbone?

26 April 2016 5,329 3 View

How to get rid of the jumps in rmsd calculation in gromacs??

I used the command g_rms -f ../run01/run01.trr -s ../run01/run01.tpr -o rmsd01.xvg -fit rot+trans -pbc to calculate rmsd. But there are jumps in the molecule. Can someone please explain how to get...

29 March 2016 9,202 3 View

What should be the minimum qualifications to become a reviewer?

Having a MSc/ MPhil/ PhD? Number of publications?

20 June 2013 4,983 28 View

Global Tea Market and COVID-19

How Covid -19 impact on global tea market..? tea is considered as one of healthy drink for covid, is there any changes is market, drinking and purchasing pattern of tea in global market

01 January 1970 1,203 8 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View

Could anybody provide insights the best tools to create a custom database for taxonomy assignment of metagenomic data?

I am interested in the different tools that can be used to create custom databases for targeted sequencing and how to trim the databases based on the amplicon size? Also, should custom databases...

02 December 2020 3,105 3 View

Does Shannon index represent largest diversity index when the individual belongs to different species and vice versa?

The shannon wiener index and Simpson indices include the measurement of community heterogeneity. Shannon wiener index bapplied information theory. The main measuring object of information theory...

22 October 2020 1,053 7 View

Is the first clean up necessary in Illumina MiSeq Library Preparation?

I'm trying to streamline my metagenomics protocols for my lab. I have previously followed the 16S protocol...

16 September 2020 5,234 6 View

How to use Vegan::capscale to calculate and plot distance based- redundancy analysis (db-RDA) and add correlation cut-offs in the results?

I have a dataset with relative abundances of Genomes against their COG-ids. It is a large data frame with 4214 rows (COG-ids) and 1313 columns...

13 September 2020 2,334 1 View

Regarding metagenomic contamination?

We have lab established culture setup of marine synechococcus PCC 7002 sp. and heterotrophic bacteria. Recently we did metagenomic analysis of it (sequencing was done by an outside reputed...

12 August 2020 187 11 View

How to find complete protein sequences (not partial) from NCBI database?

I want to generate a custom database of translated proteins involved in a particular process and then blast them against my predicted protein sequences from the metagenome . I have downloaded all...

12 July 2020 2,651 1 View