I am trying to analyse IPSC derived neurones in the seahorse bioanalyser XF24 but I am encountering many problems.

1. I am coating the plates with Poly D Lysin and Laminin - but often during the machine analysis the cells detatch.

2. as the neurons grow, they ramify and this creates gaps between the cells in the wells. The seahorse sensor has difficulty analysing the cells due to the gaps.

Has anyone else encountered these problems/can offer any advice?

thank you