What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

Which drug is better to modify blood pressure in awake monkey, if I provide it in the food or drink reward?

I need to modify the blood pressure in a monkey while is awake in the recording chamber, and I am planing on provide a drug for that in the food or water used for reward... any advise? Thank you

08 February 2021 9,900 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

Using a nucleosil c18 column, i get no negative peaks in real time and two negative peaks in post run.Software problem?

Simadzu, lc solutions

02 February 2021 1,969 2 View

Any ideas on fast and easy way to stain mucus on intact cell layer?

Dear RG community, We are looking for a fast and easy way to stain mucus on a cell layer that allows for macroscopic visualization of mucus distrubution. Our epithelium is grown in conventional...

31 January 2021 6,790 2 View

Is there a package in R that will allow me to run an AMOVA that will look at spatial versus temporal variance?

I am trying to establish a genetic monitoring program for an endangered fish species. The genetic diversity baseline was set 5 years ago. My study is a follow-up looking at impacts of a changing...

03 November 2020 4,228 5 View

How to interpret data in .arp file?

Hey, I am working in population genetics. I used DNAsp to convert fasta into .arp file for input in Arlequin. Can anyone help to interpret the data in the .arp file? As in which column represents...

12 October 2020 8,443 9 View

Are there R packages able to read genetic datasets in .arp format (Arlequin)?

Hi all! Hope you had a great summer! I was wondering if anyone knew an R package able to load genetic data from .arp files (that is, the Arlequin input file format,...

04 September 2020 6,175 2 View

How to calculate the Garza-Williamson index?

Hello, I'm trying to calculate the Garza-Williamson index and some articles indicate the Arlequin program, but there is not this option in the program. Someone already calculated this index and...

22 April 2020 8,778 1 View

Why AMOVA test is not active in Arlequin?

I entered the data to Arlequin and under Genetic structure, the AMOVA option is not active, is this related to input data? The other options are active and will run the analysis. I enclosed the file.

15 August 2019 4,564 4 View

Why AMOVA test is not active in Arlequin?

I entered the data to Arlequin and under Genetic structure, the AMOVA option is not active, is this related to input data? The other options are active and will run the analysis. I enclosed the file.

07 August 2019 1,462 4 View

In AMOVA analysis, can the variation be more among population as compared to within population?

During my AMOVA run, i got much higher genetic variation among population (69.39%) and less variation within population (30.61%). i am little confused with my result, can anyone suggest me any...

05 June 2019 3,916 2 View

Fail to use the Arlequin to do mismatch distribution?

I did mismatch distribution analysis on a population named popA in DnaSP and it succeed, but when I used Arlequin to do the same analysis, I can't get the result, and the file wrote "mismatch...

27 March 2019 8,435 8 View

Calculate Multilocus FST Software?

Hi together, I'm having a total blonde moment here, but for an assignment in University I have to compare different populations. For this I was given Genotypic data for one population. I...

12 March 2019 6,522 1 View

Fail to use the Arlequin to do mismatch distribution?

I did mismatch distribution analysis on a population named popA in DnaSP and it succeed, but when I used Arlequin to do the same analysis, I can't get the result, and the file wrote "mismatch...

02 March 2019 1,435 3 View