I am trying to run AMOVA and Fst using Arlequin with a aligned FASTA. I generated output.arp file using Rstudio and then uploaded as a project on Arlequin, however, my output file (output.xml) shows an error saying

“#[ERROR # 1] : invalid DNA data

#[ERROR # 2] : unable to read sample data

#[ERROR # 3] : unable to read sample data”

My alignment file has ATGC nucleotides and are all clean sequences.

Does anyone know what could be wrong with the files? Please see attached

Thanks

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