Hello!
I am preparing to conduct a quorum sensing signal molecule assay on a bacterial species, where I would like to characterize the secretome of n-acyl-homoserine lactones (AHLs) that this species utilize.
I will be using RPC C-18 TLC assay. For this, I will have to synthesize AHL standards, which I will apply on the TLC next to the supernatant extract to be able to evaluate on the composition of AHL in the sample.
Since I will be synthesizing a bunch of these compounds C4-HSL, C6-HSL, C8-HSL, C-10 HSL, C12-GHSL their oxo-derivatives and other) and the starting materials are not so cheap, I was wondering, what is the minimal amount of those to successfully use on a TLC? Should be pretty small I assume?
Thanks!
Artur
Dear Artur,
According to some paper using AHL, It seems that the natural concentration secreated by bacteria (e.g. Chromobacterium violaceum) is about 1 μg/ml or lower (1.21 μg/ml 6C-HSL seems enough to induce QS).
Hope these two paper would be helpful.
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