I have a DNA fragment, less than 20 nt, containing ammonium as counterion. Is it possible to exchange with a different one, like potassium?
One possibility could be an extensive gel filtration with pure water, then add KCl. Does this work?
Alternatively I heard that ammonium carbonate is volatile: what happens if I add potassium-carbonate in my DNA-ammonium sample and try to remove by heat the ammonium-carbonate?
Marcia Moss
I think adding potassium carbonate followed by vacuum maybe without heat would work for you. Thumbs up for your own idea. Alternatively, if you stick it to an ion exchange column, you can elute with potassium carbonate, but I think this is unnecessary.
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