Alternative to trypsin

More Karthik G Vaidya's questions See All

How can I isolate cell products deposited on the glassware?

Say I've grown cells on a petri plate and they have deposited their extracellular matrix onto the petri plate. Say I've decellularised it and washed away all the cells. Now is it possible to...

01 February 2019 3,317 3 View

What is the procedure for production of absorbable PGA or PLA sutures?

I wanted to know the procedure of production of absorbable sutures (PGA or PLA) including their composition and concentration of the polymer. I also want to know the time it takes to decompose in...

31 December 2018 1,792 0 View

Electrospinning in Bangalore

I want to Electrospin a polymer embedded with an antibiotic. Where, in Bangalore, can I find a Electrospinning machine ? And how do I contact them?

05 June 2017 2,797 4 View

Is there any other method to completely remove dna from a tissue other than digesting them with endonucleases and exonucleases?

I want to completely remove the dna from an animal tissue. Can it be done without using endonucleases and exonucleases? Also I was thinking of breaking the cells with a surfactant, adding chilled...

04 May 2017 7,408 2 View

How to extract Extracellular Matrix?

How can I extract extracellular matrix from a pig's bladder? Is there any other source or an easier option?

31 December 2016 3,984 2 View

What happens when excess of Extracellular Matrix Patch or Powder is used to treat a wound?

I heard about Extracellular Matrix Patch and Powder being used for treating wounds and some also being used in surgeries, I wanted to know what happens when excess of it is used? For example: I...

11 December 2016 9,865 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to draw the five chelate rings of [Co(EDTA)]- on this picture?

Is this circle one of the rings?

03 August 2024 5,988 0 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View