How can I isolate cell products deposited on the glassware?

Say I've grown cells on a petri plate and they have deposited their extracellular matrix onto the petri plate. Say I've decellularised it and washed away all the cells. Now is it possible to...

01 February 2019 3,287 3 View

What is the procedure for production of absorbable PGA or PLA sutures?

I wanted to know the procedure of production of absorbable sutures (PGA or PLA) including their composition and concentration of the polymer. I also want to know the time it takes to decompose in...

31 December 2018 1,760 0 View

Is there any other method to completely remove dna from a tissue other than digesting them with endonucleases and exonucleases?

04 May 2017 7,383 2 View

How to extract Extracellular Matrix?

How can I extract extracellular matrix from a pig's bladder? Is there any other source or an easier option?

31 December 2016 3,964 2 View

What happens when excess of Extracellular Matrix Patch or Powder is used to treat a wound?

I heard about Extracellular Matrix Patch and Powder being used for treating wounds and some also being used in surgeries, I wanted to know what happens when excess of it is used? For example: I...

11 December 2016 9,841 2 View

Alternative to trypsin

Can I use 0.05%EDTA or 0.05%NaN3 instead of 0.02%Trypsin to remove a few proteins from my test tissue? If so then what will the difference in the result that I get be? Are there any alternatives...

01 January 1970 8,629 2 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

How to do a surface treatment of uncoated TiO2 with polymer?

Basically, I would like to know how to treat or coat on the uncoated bare TiO2 (Ti-OH rich) surface with polymer which has anchoring groups in the polymer structure like trimethoxygroups. What is...

21 February 2021 4,657 6 View