Does anyone indicates a site or a book of "Meta-analysis for dummies"?

24 February 2021 2,755 4 View

Do you think the people can change his activity behavior just because have a chronic disease?

Along several years specialist study the physical activity, bur why not everybody meke changes.

03 December 2020 2,191 3 View

How to reproduce the sub-grade of pavements without using soil?

Dear colleagues, In order to evaluate the performance of concrete slabs-on-grade, the sub-grade should also be considered in the testing setup. Using soil or sand to reproduce the sub-grade is a...

20 November 2020 7,921 1 View

How is vertical agriculture progressing in Colombia?

Vertical agriculture offers an innovative solution to reduce the pressure of cultivation on soils. The intensive use of machinery and large areas of land harm biodiversity.

28 October 2020 8,773 3 View

Bioinformatic tools to determine ploidy in microbial genomes?

Looking for tools with proved accuracy to detect ploidy levels in microbial whole-genomes.

14 October 2020 4,273 3 View

How can I determine which dye I'm dealing with?

I am currently analyzing the composition of an unknown solution. It is a blue dye solution, but there are an awful lot of blue dyes. I have already analyzed the UV-VIS spectrum, the wavelength is...

08 October 2020 4,651 4 View

How to protect intellectual property rights in the innovation process?

Not all companies have an R&D area or specialists that supply their need for knowledge and technology, and in this case, they seek solutions external to organizations, through specialists or...

21 September 2020 7,918 30 View

Recommend antibodies that works in paraffin sections for synaptic and astrocytes markers in rat samples?

Hello, I am trying to find antibodies that can be used in paraffin rat brain sections against S100B , PSD95, Synapsin-I, VGLUT1,VGLUT2. I am checking the technical info provided by some companies...

12 August 2020 5,382 2 View

Innovation, Ideas permeability. Which factors make an idea to be commercialized?

There are a lot of new ideas in different fields, but only few will be innovative (new+comercialiced). As an example, Escalators and moving walks have the same mechanical design from more than...

13 July 2020 7,807 12 View

What is the minimum viral load that can be detected by TEM or SEM?

Imagine SARS-COV-2 in a surface to be recovered with an hyssop .

08 July 2020 1,193 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

SSR primers are species specific or we can use it for any plant species ?

Hi, any one please guide me; SSR primers are species specific or trait specific. i have few primer which was reported in articles for wheat yield can i use these similar primer for lentil yield...

20 February 2021 315 4 View

Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

Consensus sequence from ClustalX?

I am doing multiple sequence alignment to design primer for my gene. Can anyone tell me how to select the consensus sequence after alignment. I am using ClustalX for the alignment.

04 February 2021 8,872 2 View

What is the suitable buffer for this protein ?

I am trying to find a suitable buffer for storage of a His-tag purified protein. The amino acid sequence is pasted below. Can anyone help please? MMSFVSLLLV SILFPAIQAK QFTKCELPQV LKDMDGFGGI...

01 February 2021 5,216 4 View

Where can I find the receptor binding domain (amino acid sequence) of ALL pathogenic viruses?

I want to compare the RBD of all pathogenic viruses? Is there a database or manuscript that anyone knows of where I can access these?

25 January 2021 3,843 4 View

Luciferase assay for miRNA-gene: How to obtain DNA (PCR product) from the mutated primer?

To perform the Luciferase assay for microRNA-gene interaction its necessary a primer (wild-type) to amplificate the specific ligation site and also a mutated primer. But if your primer has a...

19 January 2021 8,568 3 View

What Contaminates Cause Loss of Resolution in Plasmid Sanger DNA sequencing?

We use an AB 3130xl Genetic Analyzer for Sanger Sequencing, and on occasion, we see our plasmid samples struggle with wavy, loss of resolution (as seen in the attached image). Our general...

14 January 2021 7,346 6 View

Why this SSR primer show band like that ?

Each time this SSR primer show band like that other show one or two band . Is it polymorphic ?

11 January 2021 2,221 5 View

Do I need to make a special plasmid for bisulfite methylation test?

I have an inducible expression of the enzyme in a newly derived bacterial strain, and methylation may be affected. I want to test methylation efficiency with a bisulfite kit. The kit is the EZ DNA...

06 January 2021 7,218 2 View