I have agrobacterium GV strain culture that were grown well on LB agar plate with gentamycin and rifampycin antibiotics. But when I reinnoculated from grown cultures plate into fresh LB broth there are no sign of growth. I even did try to inoculate the culture just on to LB broth with no addition of antibiotics, but i didn't see any growth sign yet.

I also prepared fresh medium with appropriate antibiotics to find out is there any problem with medium. I have also innoculated different cultures available in our lab (like Agrobacterium LBA strain and they grows well with appropriate antibiotics, or at least with the rifampycin). Does anyone know what is the problem and how to fix it? Thank you