I am wondering that which technology is better to find out the direct linkage between a microRNA and its mRNA targets?
Dear Researchers, I am wondering which criteria should be followed to consider a miRNA as a true miRNA. I have read few papers in which they have considered miRNA as a real miRNA even with one...
09 October 2017 5,976 6 View
Dear researchers, I am wondering why there are so many miRNA targets in coding sequence region rather than 3'UTR in an invertebrate model? According to previous reports, the target site should...
07 August 2017 9,457 2 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?
24 February 2021 7,064 3 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View
Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...
22 February 2021 9,969 3 View
I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...
22 February 2021 1,853 3 View
Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...
21 February 2021 6,633 3 View
Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...
18 February 2021 1,518 1 View
There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...
18 February 2021 9,109 5 View
I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...
17 February 2021 8,986 2 View