Please I urgently need assistance on what concentration (Unit/ml) of the enzyme, ACE (using HHL as substrate) that can be used in a spectrophotometric detection (quantification) of the product (hippuric acid) formed.I only have 0.1UN of the ACE (obtained from Sigma) for the over all assay.
However, some authors have used as low as 0.5 and 3mUnit/ml of the enzyme using HPLC method of quantification.
But I am Using spectrophotometric Method.Thanks for your kind assistance