Abraham Abbey Paul

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Questions related from Abraham Abbey Paul

Cleaning of Stained magnetic stirrer and ceramic mortar and pestle?

Please treatment would you recommend for my mortar and pestle as well as the magnetic stirrer that got stained (with black) after using them for magnetic nanoparticles synthesis? Thank you

26 May 2022 3,492 6 View

How can I quench the fluorescence of GFP by protease action?

Hi Everyone! Please can anyone recommend to me what protease(s) I can use to proteolyze my green fluorescent protein (GFP), that can lead to its loss of fluorescence? Trypsin and chymotrypsin were...

28 November 2021 1,661 3 View

Agiotensin Converting Enzyme, ACE Unit/ml?

Please I urgently need assistance on what concentration (Unit/ml) of the enzyme, ACE (using HHL as substrate) that can be used in a spectrophotometric detection (quantification) of the product...

22 March 2019 2,463 0 View

Spectrophotometric Determination of angiotensin-I converting enzyme (ACE) inhibition?

Please I urgently need assistance on what concentration (Unit/ml) of the enzyme (using HHL as substrate) can be used in a spectrophotometric detection (quantification) of the product (hippuric...

22 March 2019 7,740 3 View

How do I perform protein hydrolysis using Chymotrypsin and Trypsin together in a reaction medium without auto digestion of the enzymes?

Please I need advice. I want to perform Peptic hydrolysis of a protein sample using alpha-Chymotrypsin and Trypsin together, but my concern is, since the two enzymes are protein, would they not...

09 July 2018 8,441 2 View

Is it necessary to use either Leucine or Isoleucine in a Ninhydrin amino acid standard curve or any other amino acids excluding proline can be used?

I want to determine the degree of hydrolysis using Ninhydrin method, can i generate calibration curves for proline and any other amino acid or it must be Leu or Ile? because most researchers use...

12 May 2018 9,591 0 View

I need protocol on How to isolate angiotensin 1 converting enzyme from rabbit?

How can I isolate and characterise ACE from rabbit

19 November 2017 6,992 0 View