i was wondering if you know of an accurate method for quantifying the amount of cellulose in a filtrate. of biomass that had been dissolved in ionic liquid and then filtered with deionized water.
- how may i find out how much of cellulose is in this filtrate?
could i use fluorescence or size exclusion chromatography and if so may you please send me a method.
Chloe:
How about you weigh the weight of the filter before filtration and then dry the filter afterward, then weigh the dried filter to get the difference? If you have more than enough cellulose in the filter.
Dear Chloe,
the method you should use depends how clean the cellulose is. If it contained also lignin then using FTIR would be good option as with FTIR cellulose and lignin content can be caluslated from the ratio of certain peaks. See e.g the following article for method: D. Koutsianitis et al. / Ultrasonics Sonochemistry 23 (2015) 148–155.
But if you are curious about the dissolved cellulose in the solution (as far as I understand your question) then using KMnO4 oxidation and titrating by conventional methods would be appropriate as used for pulping samples.
Hope this helps
Best regards,
Tamás
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