In a OLS regression, how can I perform an interaction when one of the terms follows a quadratic function?

Hi everybody, One of the predictors (A) of my dependent variable clearly follows a quadratic function (A and A squared both significant). I want to see how this predictor interacts with another...

18 February 2021 4,088 7 View

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Hello, I am using Coulomb 3.3. When I try to use the option "Build input from CMT or focal mech. data" I have the next errors: Unrecognized function or variable 'deg2utm'. Error in distance2...

18 January 2021 4,487 4 View

What RNase-free solution can I use to transport fresh mice brains?

I will be having freshly isolated mice brains shipped. However, these brains will be eventually used for RNA isolation and immunofluorescence staining, so it is critical that I have them delivered...

07 October 2020 1,343 3 View

What is the recommended maximum length of listening tasks for primary school children?

I would like to know if there are studies that investigated how long primary-school children are able to concentrate on a listening task. Are there official recommendations for a maximum task length?

20 September 2020 1,934 7 View

When does speaker normalization develop in children?

I am on the research for studies that investigate speaker normalization in children. For example, I wonder whether children around the age of six years can already normalize acoustic differences...

16 September 2020 9,907 7 View

How can I prevent Ngn2 neuron-astrocyte co-cultures from peel off the coverslip?

Hello everyone, I've been co-culturing hiPSC-derived Ngn2 neurons with human fetal astrocytes, and at the time of grabbing the coverslip from the plate (24 wp) the cells peel off the coverslip. I...

03 September 2020 2,947 1 View

How can I get individual rarefaction on PAST4 (Paleontological Statistics software)?

Hi, I'm currently working with PAST 4.01 and I have my data organized on a table of 4 columns (treatments) and 45 rows (different bacteria genders) with the amount of genders found in every...

31 March 2020 1,914 1 View

Benjamini- Hochberg correction, which p value use?

We have carried out 6 repeated measures ANOVAS: 2 (group) X 4 (image categories: pleasant, unpleasant, food and neutral) for 3 physiological measures (blink, corrugator and zygomatic) and 3...

20 November 2019 3,451 3 View

Is anyone interested in evaluating the antibacterial or antifungal activities, or the toxicity of their new nanomaterials or nanoparticles?

In the Nanobiology Laboratory we are looking for international collaborators interested in evaluating the effect of the nanomaterials synthesized by them, in different model organisms, prokaryotes...

06 November 2018 1,854 7 View

What is the minimum amount of seawater necessary to obtain RNA signal?

13 September 2018 5,730 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View