What is the correct way to standardise a western blot for a cell sample that are dramatically different in size due to different growth mediums?

I am trying to quantitate the amount of a certain protein that is produced in MSC cells that are grown in two diffrerent media/serum formations. My issue is that the cells have dramatically...

15 April 2018 271 1 View

FlowJo events on the chart edges?

29 April 2016 2,840 7 View

Cell line pathogen testing?

I would like to test cells for pathogens prior to injecting them into mice. I am aware of diagnostic laboratories offering this service, but they are quite expensive. Are there any commercially...

07 March 2016 1,843 1 View

Does anyone know of software that can counterbalance subject allocations for multiple factors?

27 January 2015 8,014 2 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

No title

23 February 2021 4,014 3 View

How to improve dissolution of neosome?

I have made neosome from herbal drug. Neosomes are stable. But I face problems in dissolution. I used minimum ratio of 1:1:0.25 ( Drug:span 60: Cholesterol and GMs. Now how can I active better...

17 February 2021 3,584 1 View

Remove excess hydrazine hydrate?

How can I get rid of excess hydrazine hydrate 99%?? The reaction is converting an ester into hydrazide , required excess amount of HH to be done with ethanol as solvent. I removed ethanol by...

30 January 2021 7,753 4 View

Please share some research papers on the synthesis of Mn3O4 nanoparticles via Hydrothermal method by using Manganese(II) nitrate hydrate?

05 January 2021 9,040 4 View

How can I dissolve Sodium carboxy methyl cellulose powder in Normal Saline solution and water?

I need this solution for my research work. I am facing problem in dissolution of CMC powder

30 December 2020 5,157 2 View

How to distinguish thermal cracks due to hydration in concrete from cracks caused by sulfates under the SEM (at zoom 200 micrometers and 200 nm )?

How to distinguish thermal cracks due to hydration in concrete from cracks caused by sulfates under the SEM (at zoom 200 micrometers and 200 nm )?

18 December 2020 3,120 3 View

Dissolution study on nano co-crystals ?

Hi, SO I prepared nano co-crystals and I have done a dissolution study on them, the thing is I'm getting 130% of my co crystals at the end of dissolution which doesn't make sense !! I have...

26 November 2020 8,816 4 View

How can I dissolve biphenyl in carbon-free minimal salt media effectively?

I prepared a 5X of M9 minimal salt and 1000X elemental trace and mixed these 2 solutions to produce carbon-free minimal salt media. Biphenyl was used as a carbon source but was prepped separately...

20 November 2020 5,904 5 View

Why DMEM color is changed?

Why the pink color will change to yellow, when the DMEM media is frozen? however after dissolution in room temperature, media color will change back into pink.

26 October 2020 708 2 View