Does anyone have a recommendation on a methodology to create a stable cell line with a T-to-A mutation in a protein coding gene? We should be able to use CRISPR to induce a double-stranded break followed by HDR repair with a single stranded DNA oligo, but we are also under the impression that the efficiency of this process is quite low. Any recommendations to ensure the success of the mutation are greatly appreciated!
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line
More Cory M Howard's questions See All
Similar questions and discussions