How to achieve the convergence of the solution in COMSOL Multiphysics?

Hello! Please tell me how to achieve the convergence of the solution ... In Newton's method I set 50 iterations and the finest grid, but the solution still does not converge I would be grateful...

23 January 2021 935 5 View

How to set up a PI controller for a wind turbine pitch angle control system?

Hello dear colleagues and experts! When developing a model of a wind generator, I faced the problem of adjusting the PI controller for the pitch angle. I read a lot of articles, but the PI...

27 October 2020 9,339 10 View

What is the meaning of an adjustment for confounding factors in the context of multiple regression analysis and epidemiology?

I have reformulated the question I mean a statistical approach for adjusting values using multiple linear regression which is often used in ecological studies (e.g. Urashima et al., 2020 Int J...

13 October 2020 3,142 5 View

Analysis of Neural Data?

I would like to know what are good sources (books, tutorials, references in general) for the application of Data Science to the field of Neuroscience (Analysis of Neural Data), and where could we...

04 August 2020 5,848 4 View

Best tools for analyzing ptoein sequences?

27 April 2020 2,021 3 View

Is comparing some rates among the patients with respective rates in general population appropriate in epidemiology?

Is it appropriate to compare rates of (for instance) tea consumers among patients admitted to a hospital with some disease, with rates of tea consumers in general population in the same country?...

11 March 2020 5,560 3 View

We are looking for a relation between Cartesian octupole and Mie scattering coefficients. Does anyone know the papers having an exact formula?

Dear Colleagues, We are looking for an accurate relation between the magnitude of Cartesian octupole and Mie scattering coefficients. Something very similar to formulas (8) and (9) from...

19 February 2020 3,836 4 View

What is the difference between "exact polarizability" values and values beginning with "Polar" in the end of output Gaussian file? And which units?

Gaussian output contains a string "exact polarizability" with some values and also there are some values beginning with "Polar"in the end of the output? They have different values. What is the...

13 November 2018 3,646 3 View

Hello! Can anyone please explain the difference between static and dynamic hyper(polarizabilities)?

I'm trying to perform NLO properties calculations with Gaussian and I need to calculate first hyperpolarizability. I faced in literature with static and dynamic ones. Can anyone explain the main...

12 November 2018 1,699 4 View

Could you advice a software for DNA sequence annotation in printed materials?

Sometimes you have to represent a nucleic acid sequence on paper, not as an image, but as a text with notes. Is there any suitable program (Windows OS) possible to divide raw sequence into pieces...

24 September 2018 1,939 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View