Hi!  Have you called their customer service team?  They may be able to help if you share your data with them.  Also, I'm curious- are you measuring Total or HMW Adiponectin?

Dear Jesus,

Hi, would you please let me know:

Concentration and OD of the standard points

OD of Blank well

OD of your unknown sample (at least 10 samples)?

Looking forward

Best

Medhi

Standard Points:

ABS

1,296  32ng/ml 

1,014 16ng/ml 

0,479 4ng/ml 

0,322 2ng/ml 

0,228 1ng/ml

Blank: 0.064

Samples:

Sample 1--> 1,371 - Dil 1:20

Sample 1-->1,372 - Dil 1:20 

Sample 1-->1,247 - Dil 1:50

Sample 1-->1,452 - Dil 1:50

Sample 1-->1,398 - Dil 1:100

Sample 1-->1,473 - Dil 1:100

Sample 4--> 1:200 - 1,872

Sample 4-->1:300 - 1,624

Sample 4-->1:500 - 1,458

Sample 5--> 1:200 - 1,437

Sample 5-->1:300 - 1,354

Sample 5-->1:500 - 1,006

Sample 6--> 1:200 - 1,346

Sample 6-->1:300 - 1,401

Sample 6-->1:500 - 1,26