Adipogenesis: Is there any difference if I substitute insulin with my sample in the induction program (Extract+IBMX+DEX)?

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What is the application of organic solar cell with short life time ?

what is the application of organic solar cell with short life time ?

01 July 2024 6,839 0 View

I want to make a research about deployment of floating offshore wind turbine and i want to study about the locational location with the criteria?

can u suggest research frame work using ahp research method

10 May 2024 6,080 2 View

Is there a difference between testing learners and players in terms of how to score in basketball and what is it if the answer is yes?

Clarification of the question: In the case of a basketball educational test that measures the accuracy of ten repetitions of jumping shots from different areas: Is the method of calculating the...

20 February 2024 8,408 2 View

HELP ME.. Any suggestion on applying PVA hydrogel or alginate gel for solar evaporators?

I have been studying sodium alginate with nanocellulose composite hydrogel for some times. Using PPy as photothermal metarial, I manage to get striking low evaporation rate of 1.4 kg/m2/hr. I...

08 January 2024 441 0 View

Do red blood cells participate in immunity?

27 October 2023 1,676 6 View

How is diabetic nephropathy induced with streptozotocin ?

What period of administration and concentrations of streptozotocin cause diabetic nephropathy?

17 October 2023 2,720 0 View

Zno ( Zinc Oxide ) solubility ?

How to soluble Zno powder ?

30 July 2023 3,497 2 View

How to generate loop of Henle in kidney organoids?

Currently, it appears challenging to create mammalian kidney organoids with clear structures known as loop of Henle (LOH). Differences among cultures and difficulties in reproducibility may impede...

05 July 2023 1,019 0 View

Are there hazards with Molybdenum trioxide (MoO3) hazards in lab?

Are there hazards when dealing with Molybdenum trioxide (MoO3) a substance in the laboratory? What safety equipment should be worn? What are the conditions in the laboratory that must be...

01 July 2023 1,646 1 View

Is Root Average Square Error (RASE) same as Root Mean Square Error (RMSE) for model evaluation?

I'm using statistical software JMP to run several machine learning models viz. SVM, boosted tree and bootstrap forest. By default from the software, RASE is used to evaluate the generated models....

07 June 2023 7,546 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View