Hi everyone,
I have adhesion problems to flask when I thaw the Beas-2b cells, someone has any advice for me? someone would be willing to give me a aliquot?
Thank you
Tommaso
Hi Tommaso,
I'm not sure that you still have the problem.
How long did you allow the cells to adhere or to proliferate following the first thawing? Did you use an attachment factor?
You must absolutely use attachment factor and should wait about 10 to 15 days after first thawing and replace the medium once in 3-4 days.
Hi Vedat,
thaks for the informations.
Usually i don't use attachment factor, but i can try!
thanks you
Hello Aubrey,
Thanks for your suggestions.
tommaso
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