01 January 1970 3 6K Report

Hi. I am trying to access this publication titled "Growth in Fishes" by H Dutta, 1994. Unfortunately, our dept. does not have access. Would it be possible for anyone to help out with this, please? Thank you.

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PCR not working. Possible reasons?

I genotype and sequnce zebrafish using a bit of the fin. Lately though, I have been struggling with PCR. So far I have tried the following but to no avail: 1. Tried the primer pair in question...

07 August 2018 7,657 5 View

What is the correct term to describe mutants with both gene copies mutated but at different regions?

Following CRISPR mutagenesis and sequencing analysis of the F1s, I have found a heterozygous male with 10bp deleted on exon1 and a heterozygous female with 5bp deleted on exon 1. I was wondering...

02 March 2017 3,709 8 View

What is a good strategy to investigate whether a mutation is hypomorphic or null?

I have a mutant strain of zebrafish wherein the mutation is considered to be directly affecting hormonogenesis. My fish do show some phenotypic aberrations due to this but otherwise seem healthy...

31 December 2016 7,938 8 View

Can PBS be used as a substitute for phosphate buffer?

I need to do an ELISA assay of a hormone on some tissue samples from zebrafish. Existing literature suggests homogenising tissues in ice cold phosphate buffer. However, I found out that PBS,...

08 September 2016 3,326 4 View

Can the same ELISA assay kit for a hormone work across all species?

I am testing zebrafish and Xenopus laevis for reduced hormone levels. It is the same hormone in both species. My method of choice is ELISA. However, when I researched for the available kits, they...

07 August 2016 4,474 3 View

Has anyone used Levothyroxine for treatment of hypothyroidism in an animal model?

I have a mutant zebrafish strain that likely has hypothryoidism as a result of the mutation. I am basing this on the fact that the adults are dwarfs compared to wild type fish and also do not...

04 May 2016 7,331 2 View

How can I purify total RNA?

I just did a total RNA extraction using TRIZOL and the 260/230 values of all samples is between 0.50 and 0.90! I also noticed that for some of them the pellet did not dissolve completely. Also, I...

03 April 2016 5,567 7 View

What is the best technique to determine the genotype of an organism??

Hi. I've been using fin clips from zebrafish for genomic DNA extraction, followed by PCR, and then ExoI and TSAP treatment. I then send these off for sequencing. So far I've been using this method...

01 February 2016 2,022 4 View

What determines "heated lid" temperature?

During PCR, is there a specific parameter which helps determine the temperature of the heated lid or is this always a fixed value? For example, if I want to do a reverse transcriptase PCR where I...

01 February 2016 4,546 8 View

How does one interpret bands in negative controls for PCR?

I did a PCR for cDNA from 3 different tissues, 2 of which do not express my gene of interest. While only the predicted tissue showed the band for my gene of interest, the negative controls for...

31 December 2015 5,571 10 View

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