Access to publication on Gerontology/Karger?

More Kunal Chopra's questions See All

PCR not working. Possible reasons?

I genotype and sequnce zebrafish using a bit of the fin. Lately though, I have been struggling with PCR. So far I have tried the following but to no avail: 1. Tried the primer pair in question...

07 August 2018 7,814 5 View

What is the correct term to describe mutants with both gene copies mutated but at different regions?

Following CRISPR mutagenesis and sequencing analysis of the F1s, I have found a heterozygous male with 10bp deleted on exon1 and a heterozygous female with 5bp deleted on exon 1. I was wondering...

02 March 2017 3,845 8 View

What is a good strategy to investigate whether a mutation is hypomorphic or null?

I have a mutant strain of zebrafish wherein the mutation is considered to be directly affecting hormonogenesis. My fish do show some phenotypic aberrations due to this but otherwise seem healthy...

31 December 2016 8,055 8 View

Can PBS be used as a substitute for phosphate buffer?

I need to do an ELISA assay of a hormone on some tissue samples from zebrafish. Existing literature suggests homogenising tissues in ice cold phosphate buffer. However, I found out that PBS,...

08 September 2016 3,437 4 View

Can the same ELISA assay kit for a hormone work across all species?

I am testing zebrafish and Xenopus laevis for reduced hormone levels. It is the same hormone in both species. My method of choice is ELISA. However, when I researched for the available kits, they...

07 August 2016 4,597 3 View

Has anyone used Levothyroxine for treatment of hypothyroidism in an animal model?

I have a mutant zebrafish strain that likely has hypothryoidism as a result of the mutation. I am basing this on the fact that the adults are dwarfs compared to wild type fish and also do not...

04 May 2016 7,484 2 View

How can I purify total RNA?

I just did a total RNA extraction using TRIZOL and the 260/230 values of all samples is between 0.50 and 0.90! I also noticed that for some of them the pellet did not dissolve completely. Also, I...

03 April 2016 5,690 7 View

What is the best technique to determine the genotype of an organism??

Hi. I've been using fin clips from zebrafish for genomic DNA extraction, followed by PCR, and then ExoI and TSAP treatment. I then send these off for sequencing. So far I've been using this method...

01 February 2016 2,135 4 View

What determines "heated lid" temperature?

During PCR, is there a specific parameter which helps determine the temperature of the heated lid or is this always a fixed value? For example, if I want to do a reverse transcriptase PCR where I...

01 February 2016 4,694 8 View

How does one interpret bands in negative controls for PCR?

I did a PCR for cDNA from 3 different tissues, 2 of which do not express my gene of interest. While only the predicted tissue showed the band for my gene of interest, the negative controls for...

31 December 2015 5,709 10 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Is Galaxy.org good to use for research for analyzing data and for publication?

Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)

06 August 2024 6,610 4 View

How to convert a privately loaded document into a public document?

I attempted to make a privately uploaded text public but a window appeared that said an error occurred. There was no explanation provided as to why there was an error or what might be done to...

05 August 2024 8,025 7 View

How to send my account link and how to make my account public?

Nothing

01 August 2024 755 1 View

How can productivity (using the Google form link below to provide your answers) be achieved in manuscript publication?

Survey on Productivity in Journal Manuscript Publication Survey Form Link: (https://forms.gle/YRVrn8dL4WZJJ79S8 ) Dear Researcher, We kindly invite you to participate in our survey focused on...

29 July 2024 4,116 1 View

How can I find writers for drafting, submitting, and publishing papers?

Looking for paper collaboration writers： Collaboration Model 1: 1.1 Based on the chosen topic, complete the paper writing, select an SCI or SSCI Q1 journal, submit using my QRCID, and complete...

27 July 2024 8,965 1 View

Could someone please provide a list of journals that accept the application of methodologies in nature-based solutions?

I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.

23 July 2024 6,444 2 View

"Hello, I am trying to find public datasets containing FTIR spectra of blood samples (both healthy and disease-related)?

These datasets will be used in the training of machine learning algorithms. Does anyone know any available data?"

17 July 2024 6,519 3 View

You have mentioned as a co-author of one publicaitron?

You have mentioned as a co-author of one publication, but it is not exactly a publication , it is an abstract submitted to: Congresso Brasileiro de Hematologia, Hemoterapia e Terapia Celular -...

17 July 2024 4,035 15 View

Who wants opportunities for scientific cooperation?

Dear Colleagues, I hope this message finds you well. My name is Noor Al-Huda K. Hussein, and I am a researcher specializing in deep learning applications in genetic data analysis. I am currently...

16 July 2024 3,981 6 View

Emad Kamil Hussein

https://www.researchgate.net/project/Researcher-Skills-Improvement

Karishma Chhabria

Hi Kunal,

Did you find the article already? I tend to use booksc.org for the articles I cant find. Attached is your article in case you didn't find it yet.

Cheers,

Karishma

Kunal Chopra

Thanks, Karishma! Two others also sent me a copy of the article!