Any suggestions will be much appreciated.
Since the cells have been cultured for many passages it does not have to be passed through a cell strainer. A quick PBS (CMF) wash should be sufficient to seperate the clumped cells.
Ashutosh Acharya thank you very much for your answer.
depends on the size of your cells, 50 or 20 mikrometers should do for epithelial cells
Hi, I am trying to perform mesh convergence and I found out there is no stable temperature value I obtained. So, I calculated the skin depth and it was around 0.00011mm. It is so small in...
24 August 2020 9,060 2 View
Hi I wanted to model Induction heating in comsol. Apply a current of 600A for 10 minutes in my coil and then a 100A current for 5 minutes. I could not find much of tutorials. I do not know where...
10 April 2020 5,565 12 View
Hello, i hope you all are good. i am modeling a system for charging and discharging cost minimization for different EV (Electric vehicle) agents like households, business...
27 November 2019 5,599 4 View
1) I am trying to plot the Basin of attraction for 2d discrete dynamical system in MATLAB. Please give some suggestions to get them. 2) If possible, give some simple example like...
08 November 2019 5,668 3 View
Hello. I had a run error *7007* that the allocated memory is 17,500 Mb but the run for the model required 18,400 Mb. When i check my directory file, there is more memory around 1TB and the system...
06 November 2019 8,994 3 View
Hello all, I need a help really. I have displacement at a point as in the image where it shows Mag = 0 and Z = 0. Apart from static min and max. That nodal point on top I needed the values. Since...
03 October 2019 3,670 3 View
A bracket has been analyzed and the solid is not flat. The triangular elements are bumpy on 2 sides of the bracket and I want it to make it flat before I re-analyze for static displacement and...
16 September 2019 3,885 3 View
Need reviews regarding measurement of aggregate stability.
14 March 2017 9,709 2 View
Current statistical data on acreage of different forages in India.
14 March 2017 5,084 2 View
Please suggest me the precise and standard methodology to compute the various N fractions in rhizospheric soil samples.
09 March 2017 6,447 6 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...
03 March 2021 961 3 View
02 March 2021 3,060 3 View
Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...
02 March 2021 1,757 4 View
I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.
02 March 2021 1,516 4 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
01 March 2021 3,905 5 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View