About What pore size cell strainer should I use to obtain a single cell suspension ?

More Adamska Mr's questions See All

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,726 6 View

Are those seeking solution part of the problem?

In reference to antibiotic as a commodity, what is your take and way forward on common molecules in both Veterinary and Human antibiotics in relation to currently experienced emergency of AMR?

20 July 2024 4,020 0 View

Is there any formula which is used to calculate the value of capacitive contribution in the case of battery type electrode?

Dunn's equation should not be used in the case of a battery-type electrode, according to a paper published in the journal "Phys. Chem. Lett. 2020, 11, 9846–9849 (ref. attached)" that I recently...

12 May 2024 3,261 0 View

Does multivariate NeuralProphet need future regressor to predict the future ?

Hello everyone,I am currently working with the multivariate time series forecasting by using NeuralProphet to predict the price of product.I used 7 economic indicators as regressor in my...

22 March 2024 8,264 3 View

I would like to know that prophet time series forecasting is under the category of machine learning or neural network?

I would like to know that prophet time series model is under the category of neural network or machine learning or deep learning? I want to forecast the price of product depending on other...

19 February 2024 1,242 1 View

How to do Strain controlled cyclic triaxial testing using GDS ELDYN triaxial set up?

Dear RG users, I hope all of you are doing well. I just wanted to know how can I do dispacement controlled cyclic triaxial tests using ELDYN dynamic equipment. As you can see in the figure, there...

14 September 2023 2,217 0 View

How to find the contributions of electronic and vibrational?

generally polar basis set will provide the tensors (dipole moment,polarizability and hyperpolarizability). but how to find their contributions separately

24 June 2023 1,295 6 View

Why is cell conditioned medium concentrated after collection? For transplantation experiments, can it be used without concentrating it?

To study the effects of cell conditioned medium (CM) in animals in vivo, is it necessary to concentrate the medium after collection? I am not familiar with the preparation of the CM.

01 March 2023 695 3 View

Is there any model which is more suitable for contrastive learning except SimCLR and MOCOv2?

These two models take a lot of time to train with powerful GPUs and required a minimum 512 batch size for good model performance.

28 February 2023 1,654 1 View

How to calculate the depletion width (width of space charge layer) using Mott-Schottky analysis?

Width of depletion region which can be remarked using width of space charge layer (WSCL).

06 February 2023 2,621 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,822 3 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,047 2 View

Request Python code?

Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!

08 August 2024 6,241 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Stability of the Solar System: Insights from Einstein’s Equations ?

The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...

07 August 2024 2,568 1 View

Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,010 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,104 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 639 2 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 665 4 View

Ashutosh Acharya

Since the cells have been cultured for many passages it does not have to be passed through a cell strainer. A quick PBS (CMF) wash should be sufficient to seperate the clumped cells.

Adamska Mr

Ashutosh Acharya thank you very much for your answer.

Irena Srebotnik Kirbis

depends on the size of your cells, 50 or 20 mikrometers should do for epithelial cells