We know that the shaking incubator gives a large amount of the fungal growth due to agitation, mixing media with fungal elements but I would like to know :Please, does the shaking incubator effect to get the different chemical structures of the fungal secondary metabolites compared with incubator ( without shaking) for the same fungus in same conditions ( medium, pH, and temperature)?. By another hand, if anyone grows Penicillium notatum in shaking incubator by using potato dextrose broth at 25 ˚C for 7 days, and the same fungus is cultured by using the same conditions except using incubator (without shaking) instead of shaking incubator. Does the shaking incubator lead to get secondary metabolite differs in the chemical structure compared with those extracted by the incubator without shaking, and by using a same extracting solvent?

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