Hi, I have a question about mouse IL-6 Elisa assay. I am preparing for this Elisa assay, but I am not sure how much dilution that I should do for this assay. My sample is serum from mouse. According to the instruction, it is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. Does anyone know which one would be better? 10% or 50%? Thank you!
When performing an ELISA assay, the dilution of the sample depends on various factors, including the concentration of the analyte of interest and the detection range of the ELISA kit. In the case of mouse IL-6 ELISA assay, the suggested starting point for dilution is to use PBS supplemented with 10-50% animal serum. However, the specific percentage within this range should be optimized for your particular samples.
To decide between using 10% or 50% serum, you can start with a pilot experiment to determine the optimal dilution for your samples. Here's a general approach you can follow:
The optimal dilution can vary depending on the concentration of IL-6 in your specific samples. It's important to consider the detection range of the ELISA kit and ensure that your diluted samples fall within that range for accurate measurement.
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