About extraction of gDNA from yeast species?

Mohamed Sharukh Khan @Mohamed_Sharukh_Khan

04 April 2018 1 2K Report

Actually Im getting good concentration and ratio of gDNA but when I'm amplifying in gel with specific primers I'm getting no result or no expected band size. I knew I'm using correct primers to amplify but I want to know about the gDNA protocol in which 50mM EDTA is used as resuspension buffer whether it inhibits my PCR reaction or can I get the protocol for gDNA extraction with correct concentration of EDTA?

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