11 November 2016 0 3K Report

Is it theoretically possible to use avidin-biotin complex staining and immunofluorescence on the same slice? What would merged photo look like?

Thanks

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Can tissue samples be deproteinized for measurement of NAPD/NADPH with 4%w/v sulfosalycilic acid?

How will 4% w/v SSA interfere with the NADP/NADPH ratio? Can this method of deproteinization be used as an alternative to 10 kDa cut-off spin columns in order to measure NADP/NADPH ratio?

10 November 2019 3,514 3 View

What's the longest passive avoidance memory task retention time in rats you've seen in the literature?

How long after the conditioning (control) rats remember the shock. I know it depends on various factors (age of the animal, shock mA, etc.). What's the longest period you've come upon. Thanks

09 October 2019 9,331 3 View

How littermates influence novel object recognition task?

Could novel object recognition task be used with several animals at the same time in the same arena? How would interaction with littermates influence the outcome? Does anyone use more than one...

09 October 2019 2,715 4 View

Anybody knows an open-source/free virtual reality morris water maze for humans?

Is there a free software for virtual reality morris water maze tests for humans? Also, are there open arm or other tests in VR format? Thanks! Best, Jan

11 December 2018 456 1 View

Can neurofibrillary tangles (NFTs) be visualized with Congo Red?

Can neurofibrillary tangles (NFTs) be visualized with Congo Red? What would be the difference between various staining methods used for this (ie. HE, silver stainings,...)? What's the best one?...

08 September 2018 3,432 3 View

Is there a website/social network for scientific methods and protocols?

Researchgate, Figshare, Academia, Mendeley, Twitter,... there are a lot of websites currently used for discussion and promotion of scientific research. Is there a website or a social network with...

08 September 2017 3,850 0 View

C-fos immunofluorescence background difference?

Hi,For some reason I get much higher c-fos background signal in the rat brain for one animal than for the other (see attached photo). The staining protocol is the same for both specimens, as well...

07 August 2017 467 4 View

Arduino ad8232 sensor data recording frequency?

I have problems with Arduino AD8232 sensor. I use classic Arduino 1.8.2 for Arduino and Processing for visual processing of the data. According to the graph Arduino sends data in at least...

04 May 2017 9,940 4 View

Can gut derived BDNF influence the brain?

Can BDNF from gut somehow influence the brain? Can you suggest any papers on this topic? Is it rational to think BDNF could go to the brain through blood and surpass liver? Could BDNF alter gut...

02 March 2017 4,709 5 View

Diffusion blotting protocol for SDS-PAGE gels?

Do you have experience with diffusion blotting from SDS-PAGE? Would you mind sharing a protocol? Prof. Ingrid Olsen shared a great protocol for precast 0.5mm GE Healthcare SDS-PAGE gels in the...

02 March 2017 9,744 2 View

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