Is it theoretically possible to use avidin-biotin complex staining and immunofluorescence on the same slice? What would merged photo look like?
Thanks
How will 4% w/v SSA interfere with the NADP/NADPH ratio? Can this method of deproteinization be used as an alternative to 10 kDa cut-off spin columns in order to measure NADP/NADPH ratio?
10 November 2019 3,804 3 View
How long after the conditioning (control) rats remember the shock. I know it depends on various factors (age of the animal, shock mA, etc.). What's the longest period you've come upon. Thanks
09 October 2019 9,514 3 View
Could novel object recognition task be used with several animals at the same time in the same arena? How would interaction with littermates influence the outcome? Does anyone use more than one...
09 October 2019 2,901 4 View
Is there a free software for virtual reality morris water maze tests for humans? Also, are there open arm or other tests in VR format? Thanks! Best, Jan
11 December 2018 643 1 View
Can neurofibrillary tangles (NFTs) be visualized with Congo Red? What would be the difference between various staining methods used for this (ie. HE, silver stainings,...)? What's the best one?...
08 September 2018 3,617 3 View
Researchgate, Figshare, Academia, Mendeley, Twitter,... there are a lot of websites currently used for discussion and promotion of scientific research. Is there a website or a social network with...
08 September 2017 4,052 0 View
Hi,For some reason I get much higher c-fos background signal in the rat brain for one animal than for the other (see attached photo). The staining protocol is the same for both specimens, as well...
07 August 2017 684 4 View
I have problems with Arduino AD8232 sensor. I use classic Arduino 1.8.2 for Arduino and Processing for visual processing of the data. According to the graph Arduino sends data in at least...
04 May 2017 10,137 4 View
Do you have experience with diffusion blotting from SDS-PAGE? Would you mind sharing a protocol? Prof. Ingrid Olsen shared a great protocol for precast 0.5mm GE Healthcare SDS-PAGE gels in the...
02 March 2017 9,929 2 View
Can BDNF from gut somehow influence the brain? Can you suggest any papers on this topic? Is it rational to think BDNF could go to the brain through blood and surpass liver? Could BDNF alter gut...
02 March 2017 4,905 5 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?
04 August 2024 1,537 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View