Can tissue samples be deproteinized for measurement of NAPD/NADPH with 4%w/v sulfosalycilic acid?

How will 4% w/v SSA interfere with the NADP/NADPH ratio? Can this method of deproteinization be used as an alternative to 10 kDa cut-off spin columns in order to measure NADP/NADPH ratio?

10 November 2019 3,514 3 View

What's the longest passive avoidance memory task retention time in rats you've seen in the literature?

How long after the conditioning (control) rats remember the shock. I know it depends on various factors (age of the animal, shock mA, etc.). What's the longest period you've come upon. Thanks

09 October 2019 9,331 3 View

How littermates influence novel object recognition task?

Could novel object recognition task be used with several animals at the same time in the same arena? How would interaction with littermates influence the outcome? Does anyone use more than one...

09 October 2019 2,715 4 View

Can neurofibrillary tangles (NFTs) be visualized with Congo Red?

Can neurofibrillary tangles (NFTs) be visualized with Congo Red? What would be the difference between various staining methods used for this (ie. HE, silver stainings,...)? What's the best one?...

08 September 2018 3,432 3 View

Is there a website/social network for scientific methods and protocols?

Researchgate, Figshare, Academia, Mendeley, Twitter,... there are a lot of websites currently used for discussion and promotion of scientific research. Is there a website or a social network with...

08 September 2017 3,850 0 View

C-fos immunofluorescence background difference?

Hi,For some reason I get much higher c-fos background signal in the rat brain for one animal than for the other (see attached photo). The staining protocol is the same for both specimens, as well...

07 August 2017 467 4 View

Arduino ad8232 sensor data recording frequency?

I have problems with Arduino AD8232 sensor. I use classic Arduino 1.8.2 for Arduino and Processing for visual processing of the data. According to the graph Arduino sends data in at least...

04 May 2017 9,940 4 View

Can gut derived BDNF influence the brain?

Can BDNF from gut somehow influence the brain? Can you suggest any papers on this topic? Is it rational to think BDNF could go to the brain through blood and surpass liver? Could BDNF alter gut...

02 March 2017 4,709 5 View

Diffusion blotting protocol for SDS-PAGE gels?

Do you have experience with diffusion blotting from SDS-PAGE? Would you mind sharing a protocol? Prof. Ingrid Olsen shared a great protocol for precast 0.5mm GE Healthcare SDS-PAGE gels in the...

02 March 2017 9,744 2 View

Undergraduate and graduate student research grants?

Does anybody know any undergraduate and graduate student research grants in the field of neuroscience?Thanks

02 March 2017 9,158 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View