Can tissue samples be deproteinized for measurement of NAPD/NADPH with 4%w/v sulfosalycilic acid?

More Jan Homolak's questions See All

What's the longest passive avoidance memory task retention time in rats you've seen in the literature?

How long after the conditioning (control) rats remember the shock. I know it depends on various factors (age of the animal, shock mA, etc.). What's the longest period you've come upon. Thanks

09 October 2019 9,514 3 View

How littermates influence novel object recognition task?

Could novel object recognition task be used with several animals at the same time in the same arena? How would interaction with littermates influence the outcome? Does anyone use more than one...

09 October 2019 2,901 4 View

Anybody knows an open-source/free virtual reality morris water maze for humans?

Is there a free software for virtual reality morris water maze tests for humans? Also, are there open arm or other tests in VR format? Thanks! Best, Jan

11 December 2018 643 1 View

Can neurofibrillary tangles (NFTs) be visualized with Congo Red?

Can neurofibrillary tangles (NFTs) be visualized with Congo Red? What would be the difference between various staining methods used for this (ie. HE, silver stainings,...)? What's the best one?...

08 September 2018 3,617 3 View

Is there a website/social network for scientific methods and protocols?

Researchgate, Figshare, Academia, Mendeley, Twitter,... there are a lot of websites currently used for discussion and promotion of scientific research. Is there a website or a social network with...

08 September 2017 4,052 0 View

C-fos immunofluorescence background difference?

Hi,For some reason I get much higher c-fos background signal in the rat brain for one animal than for the other (see attached photo). The staining protocol is the same for both specimens, as well...

07 August 2017 684 4 View

Arduino ad8232 sensor data recording frequency?

I have problems with Arduino AD8232 sensor. I use classic Arduino 1.8.2 for Arduino and Processing for visual processing of the data. According to the graph Arduino sends data in at least...

04 May 2017 10,137 4 View

Diffusion blotting protocol for SDS-PAGE gels?

Do you have experience with diffusion blotting from SDS-PAGE? Would you mind sharing a protocol? Prof. Ingrid Olsen shared a great protocol for precast 0.5mm GE Healthcare SDS-PAGE gels in the...

02 March 2017 9,929 2 View

Can gut derived BDNF influence the brain?

Can BDNF from gut somehow influence the brain? Can you suggest any papers on this topic? Is it rational to think BDNF could go to the brain through blood and surpass liver? Could BDNF alter gut...

02 March 2017 4,905 5 View

Undergraduate and graduate student research grants?

Does anybody know any undergraduate and graduate student research grants in the field of neuroscience?Thanks

02 March 2017 9,342 3 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View