How to stain for slow vs fast muscle fibers in PFA fixed tissue?

09 August 2017 7,346 3 View

Fermi level changes with applied electric field?

Hi people, I dont understand why when we apply the electric field to the metal Ef remains the same. Ef as translation energy of electrons remains the same but we accelerate the electrons with...

08 March 2016 1,747 3 View

What does it mean the high real part of refractive index of metal?

in concept of material structure.

21 February 2016 6,918 1 View

What publications on classical mesoscopic systems can be found?

The theory of mesoscopical systems can be divided into two parts: theory of mesoscopic quantum systems and the classical theory of mesoscopic systems. In a quantum mesoscopic considered electronic...

24 December 2015 5,261 3 View

Why in derivation of the equations of continuum mechanics the model of material point is used?

Model material points cannot be used for describing the motion of macroscopic bodies. The derivation of the equations of continuum mechanics based on the use of limit theorems, in which the size...

11 October 2014 3,519 17 View

Can you use equations of motion not for material points but for equations of mesoscopic particles?

In mesocsopical systems, interactions described with action fluxes in a mechanical system are flow of impulse. (See attachments.) Lev

20 August 2014 6,438 2 View

Is there any evidence that different dendrites and/or axons in the same neuron may carry qualitatively (not quantitatively) different information?

We have proposed a theoretical model which might be interpreted as suggesting that individual dendrites and/or axons play qualitatively different roles.

03 March 2013 5,676 18 View

What is the main road leading to the development of AI and its various subfields (pattern recognition, data mining, information retrieval, etc.)?

I propose that the *only* way ahead is via a fundamentally new representational formalism that would clearly reveal what critical information has been missing from the conventional forms of data...

28 October 2012 340 13 View

Have you noticed the lack of serious *scientific* discussions on scientific internet forums (and conferences) despite the often repeated "internet opportunities"?

More accurately, I mean not the narrow technical discussions but the discussions on the general direction of the corresponding natural science. And this is particularly conspicuous given the...

24 October 2012 7,518 14 View

What is the *concept* of class in your favorite learning model, e.g. in SVM, ANN, etc.?

My claim is that none of the accepted concepts of class are *adequate*. Is this situation ok? Is this ok to do classification without an adequate (central) concept of class available in the model?...

09 October 2012 4,471 7 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How to reduce debris with neuron isolation?

Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...

14 February 2021 3,692 4 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Safety of the SARS-Cov-2 mRNA vaccine?

Dear colleagues I have the following questions concerning the safety of mRNA vaccines targeting the spike protein of SARS-Cov2: 1) The vaccine elicits antibodies which block the peptide domain...

04 February 2021 721 6 View

Has anyone used Pro-Touch color bands to mark raptors?

We have been color banding American Kestrels using A-craft alpha-numeric color bands since ~2013 and would like to test other options. I am curious if any other researchers have experience using...

01 February 2021 6,786 3 View