Does sterile filtration remove dissolved salts from solution?

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How to perform a ChIP assay from a Lizard blood?

I need to take blood from Tegu Lizard and to extract the RBC following nuclei isolation, sonication and ChIP assay: appreciate any help: 1) which blood-collection tubes are recommended? 2) how do...

03 April 2024 9,657 0 View

What antigen retrieval method is most suitable for laminin and eMHC on paraffin-embedded sections?

Hello everyone, We are struggling with fluorescence immunostaining of paraffin-embedded sections of TA muscle. We're staining with laminin, pax7 and eMHC. When we first stained I used the...

20 December 2021 8,275 1 View

Is there a Gravity model of trade tutorial somewhere?

There is a ton of articles and videos about the gravity model of trade, but they either give you the basic theory or provide a result and analysis without panel data. Is there any step-by-step...

01 May 2021 4,881 7 View

May I interest you in a cross national collaboration on this project?

Dear Professor Debra Lupton, I hope you are well, My name is Dr Shirly Bar-Lev, I am the head of the Health Informatics Research Center in Ruppin Academic Center. I am researching similar...

04 May 2020 3,439 4 View

A role for rDNA copy number as a modulating factor of general translational capacity in the translational control of neuronal activity?

Dear project members! With great interest I monitor your works. Do you think worthy to study a role for other translation factors such as rDNA copy number in the operation of neuronal networks?...

20 November 2019 4,413 0 View

How to stain for slow vs fast muscle fibers in PFA fixed tissue?

Hi, I am having trouble staining for slow vs fast muscle fibers on 4% PFA fixed mouse calf cross sections. Currently I am trying the Vector M.O.M kit with either: Abcam Anti-Slow Skeletal Myosin...

09 August 2017 7,446 3 View

Fermi level changes with applied electric field?

Hi people, I dont understand why when we apply the electric field to the metal Ef remains the same. Ef as translation energy of electrons remains the same but we accelerate the electrons with...

09 March 2016 7,202 4 View

Fermi level changes with applied electric field?

Hi people, I dont understand why when we apply the electric field to the metal Ef remains the same. Ef as translation energy of electrons remains the same but we accelerate the electrons with...

08 March 2016 1,883 3 View

What does it mean the high real part of refractive index of metal?

in concept of material structure.

21 February 2016 7,025 1 View

What publications on classical mesoscopic systems can be found?

The theory of mesoscopical systems can be divided into two parts: theory of mesoscopic quantum systems and the classical theory of mesoscopic systems. In a quantum mesoscopic considered electronic...

24 December 2015 5,370 3 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

Is it true that $\det(V(A))$ may be only $\pm 1$, depending on $n$, for the last symmetric tridiagonal matrix $A$?

One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...

08 August 2024 6,690 1 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Why is nonpoint source pollution potentially more harmful and difference between point and nonpoint sources of water pollution?

01 August 2024 1,180 2 View

How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?

01 August 2024 7,061 2 View

The use of data from PubChem for commercial purposes?

Hi, I'm curious to know if data on chemical compounds from PubChem, such as water solubility properties, can be used to train a machine learning model for commercial purposes. Will this infringe...

30 July 2024 8,707 1 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View