A question to the neurosurgeon committee?? In PVL can we replace the dead tissue by Wight matter cells to treat cerebral palsy?

09 May 2024 0 9K Report

A question to the neurosurgeon committee? ? In PVL can we replace the dead tissue by Wight matter cells to treat cerebral palsy?

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The future of stem cells in brain repair??

The future of stem cells in brain repair and TBI???

08 May 2024 8,126 0 View

Why do I get different results between Coomassie Gels and Western Blot?

Hi everyone, I hope you're doing well. I've encountered an interesting phenomenon in my research and would appreciate your insights. When running gels and staining them with Coomassie, I'm...

18 March 2024 3,416 0 View

Why am I unable to detect pERK in HEK293T cells through Western Blotting?

Greetings to all, I have attempted to detect pERK using western blotting with HEK 293T cells, but I am unable to do so. I overexpressed a gene that caused pERK to be overproduced, and I was...

21 December 2023 7,195 3 View

Inner geometry suppression when refining the mesh?

Dear ICEM CFD users, Could you please help me to solve this problem? I have a inner geometry inside an other. A coarse mesh of such a configuration captures the inner geometry but a refined mesh...

25 September 2023 2,887 0 View

Do conifers produce tracheid with spiral thickness? If not, Is tracheids spiral thickness the same thing as the "Cellulose Microfibril Angle"?

Hello, Tracheid in Cupressaceae and other conifers may exhibit ornamentation on their walls that resemble helical spiral thickness little bit, as result of an angle in Microfibrils. What is the...

22 January 2023 5,334 2 View

How can i do Mycoplasma test in tissues ?

is it possible to do mycoplasma contamination test in my Warthon jelly tissues frozen at -80°C ? all what i found n the internet is compatible with cell culture supernatant and nothing about...

30 November 2022 6,320 3 View

How do I interpret my MTT assay results, and what statistical tests should I do for cell viability?

I have completed an experiment where I exposed rainbow trout liver cells to copper concentrations of 0.01, 0.1, 1.0 M, along with a negative control. I have recorded the cell viability % at each...

20 March 2021 9,445 7 View

Can any one explain why the ethyl ester Biodiesel (FAEE) has higher than that of the metyl ester FAME- both of them are derived from the same oil?

Biodiesels derived from waste vegetable oil transesterification with Ethanol (FAEE) show higher peroxide values than those derived by transesterification with methanol (FAME) prepared under the...

13 February 2021 515 6 View

I need a help from a spring technician or some who know a lot about heavy duty spring ?

I need a spring technician or some who know a lot about heavy duty spring to ask him/her a couple of questions in my bachelor thesis. The question will be in direction of choosing the right...

20 December 2020 8,646 0 View

What happens to a gas after compressing it for 10 years?

Will it lose a fraction of its expansion power assuming it was insulated in this system? Because I am designing an insulated gas spring. what I want to know if the gas will lose some of its...

07 December 2020 6,363 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

Why is this tissue slice different?

This is a slice of intestinal tissue from the Chinese mitten crab (intestine in the upper right corner). I injected WSSV into this horseshoe crab. Now I want to know what these red spots next to...

18 July 2024 5,602 0 View

Can we progress towards defining obesity based on body fat content?

The burden of obesity is enormous. The greater challenge lies in accurately diagnosing obesity at the right time, reflecting the true increase in visceral fat. BMI may not accurately represent...

14 July 2024 8,771 4 View